The Peptide CommonsEst. May 2024
Independent. We sell nothing and are affiliated with no manufacturer or pharmacy. Every moderation action is logged in public
Analytics · subcategory

HPLC & UHPLC

Reversed-phase separations, gradients, columns, integration, and reading a chromatogram properly.

35 topics1,237 posts10 solved Moderators: KLindqvist, bench_notes About this category

35 topics · page 2 of 2

TopicParticipantsRepliesViewsActivity
Area percent versus weight percent: the confusion that causes most arguments — what changed since
Area percent versus weight percent: the confusion that causes most arguments — what changed since Writing it up because I had to work it out twice and would rather nobody else did. Posting the method first,…
VBSADMRLEN+30 34 40k 13mo
Carryover and the ghost peak from last week's standard
On the subject in the title: Carryover and the ghost peak from last week's standard Working notes rather than a conclusion. Posting the method first, because I know what the first three replies will otherwise…
PBFDJMKOHD+80 92 16k 16mo
Coming back to: Column chemistry choices for a 40-residue peptide
On the subject in the title: Column chemistry choices for a 40-residue peptide Working notes rather than a conclusion. Posting the method first, because I know what the first three replies will otherwise be.…
MLFPHB 2 43k 16mo
Why I ask for the gradient before I discuss the number
The question in the title: Why I ask for the gradient before I discuss the number I will give what I have already checked below so nobody repeats it. I would like to understand what this number means before I…
GPSFCNNDHK+35 39 4.9k 20mo
Gradient steepness and apparent purity: a worked comparison
On the subject in the title: Gradient steepness and apparent purity: a worked comparison Working notes rather than a conclusion. A documentation question rather than an analytical one. I have a certificate in…
POLFTI 2 51k 22mo