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Research Methods · N-of-1 designs · continued

Pre-registering a personal experiment, seriously — a second dataset posts 31–60

This is a continuation of a long topic, addressed by post number rather than by page. Start at post 1.

MA
m.adebayoTL2 Moderator12 Feb 2026#31

On post #27 — agreed on the reasoning, with one qualification.

Sample size in n-of-1: you are the sample. Repeated measurements (weekly weighings, daily mood scores) increase the power to detect a real effect even though n=1.

22 likes 5mo
BP
bench_peakTL3Regular15 Feb 2026#32

I disagree with the reply above, and I think the disagreement is substantive rather than terminological.

The distinction being drawn does not survive when you look at the published data for this specific question. I would be glad to be shown wrong on this, because the version I am arguing against is more convenient.

9 likes 5mo
PB
p.boatengTL2 Moderator18 Feb 2026#33
a.finnegan_rd, post #3: Washout periods: after stopping a medication, how long does it take for the effect to wash out? For compounds with a week-long half-life, roughly a month is needed to reach baseline. Using that washout period in a before-after design strengthens the inference. Go to post

Confounding in personal experiments: other things change when you start a medication (season, exercise, diet, stress). Documenting those confounders helps you understand their contribution to the result.

1 like in reply to #3 5mo
LC
l.chevalierTL3Regular20 Feb 2026 · edited#34
n.kaufmann, post #29: Washout periods: after stopping a medication, how long does it take for the effect to wash out? For compounds with a week-long half-life, roughly a month is needed to reach baseline. Using that washout period in a before-after design strengthens the inference. Go to post

Statistical analysis of n-of-1 data: comparing before versus after with a t-test or similar is one approach. Plotting the data visually is another. Both are valid.

0 likes in reply to #29 5mo
RC
r.chukwuTL2 Moderator23 Feb 2026#35

Stopping rules: decide in advance when you will stop measuring (after a defined duration, after a defined number of measurements, or after a defined condition is met). Not deciding in advance means stopping when the result satisfies you, which is bias.

29 likes 5mo
T
TavaresTL1Member25 Feb 2026#36

Designing a personal experiment that could actually change your mind: that is the standard for an n-of-1 design. An experiment designed so that any result confirms what you already believed has not changed anything.

14 likes 5mo
RM
r.mwangiTL2 Moderator28 Feb 2026#37

Having read the exchange above, I think I was wrong earlier in this topic and I want to say so plainly rather than quietly editing.

The correction was fair and I had been repeating something I had not checked carefully enough.

2 likes 5mo
B
BramleyTL2Member2 Mar 2026#38
Tavares, post #36: Designing a personal experiment that could actually change your mind: that is the standard for an n-of-1 design. An experiment designed so that any result confirms what you already believed has not changed anything. Go to post

This follows post #35 rather than contradicting it.

Objective versus subjective measures: subjective measures (how you feel) are vulnerable to bias. Objective measures (weight, strength on a specific exercise) are less vulnerable but not immune.

0 likes in reply to #36 5mo
KK
k.kuuselaTL2 Moderator5 Mar 2026#39

Confounding in personal experiments: other things change when you start a medication (season, exercise, diet, stress). Documenting those confounders helps you understand their contribution to the result.

0 likes 5mo
NR
n.rowntreeTL3Regular7 Mar 2026#40

Statistical analysis of n-of-1 data: comparing before versus after with a t-test or similar is one approach. Plotting the data visually is another. Both are valid.

21 likes 5mo
AB
a.batistaTL2 Moderator10 Mar 2026#41
Wendelboe, post #19: Blinding: blinding yourself (not knowing which condition you are in) removes expectation bias. This is hard to do with these compounds (the appetite suppression is hard to miss) but partial blinding is possible (measuring something objective without knowing whether you took it today). Go to post

Washout periods: after stopping a medication, how long does it take for the effect to wash out? For compounds with a week-long half-life, roughly a month is needed to reach baseline. Using that washout period in a before-after design strengthens the inference.

0 likes in reply to #19 5mo
KP
k.perrinTL2 Moderator12 Mar 2026#42
n.kaufmann, post #29: Washout periods: after stopping a medication, how long does it take for the effect to wash out? For compounds with a week-long half-life, roughly a month is needed to reach baseline. Using that washout period in a before-after design strengthens the inference. Go to post

On post #38 — agreed on the reasoning, with one qualification.

Two things before anyone answers the substance.

First, the context in the first post is clear and specific. Second, the question is framed so that an answer can actually address it. Both are the norm here and both matter more than they sound.

0 likes in reply to #29 5mo
BN
bench_notesTL4 Moderator15 Mar 2026#43
Staff post. Actions described here are recorded in the public moderation log and may be challenged in Meta.

Objective versus subjective measures: subjective measures (how you feel) are vulnerable to bias. Objective measures (weight, strength on a specific exercise) are less vulnerable but not immune.

4 likes 4mo
EV
e.vargaTL2 Moderator17 Mar 2026#44

Sample size in n-of-1: you are the sample. Repeated measurements (weekly weighings, daily mood scores) increase the power to detect a real effect even though n=1.

12 likes 4mo
OV
o.vogelTL2 Moderator20 Mar 2026#45
r.mcalister, post #26: Designing a personal experiment that could actually change your mind: that is the standard for an n-of-1 design. An experiment designed so that any result confirms what you already believed has not changed anything. Go to post

post #44 is right about the mechanism and I think understates the practical bit.

Generalisability: a robust n-of-1 result applies to you. It does not tell you much about whether the effect generalises to others similar to you, much less to people different from you.

0 likes in reply to #26 4mo
AZ
a.zamoraTL2 Moderator22 Mar 2026#46
r.mwangi, post #37: Having read the exchange above, I think I was wrong earlier in this topic and I want to say so plainly rather than quietly editing. The correction was fair and I had been repeating something I had not checked carefully enough. Go to post

Worth separating two things that post #42 runs together.

When to run an n-of-1: this design works when you want to know whether a treatment works for you, not whether it works in general. For that purpose, it is efficient.

1 like in reply to #37 4mo
DT
d.tammTL2 Moderator25 Mar 2026 · edited#47

Stopping rules: decide in advance when you will stop measuring (after a defined duration, after a defined number of measurements, or after a defined condition is met). Not deciding in advance means stopping when the result satisfies you, which is bias.

7 likes 4mo
AN
a.nwosuTL2 Moderator27 Mar 2026#48

Designing a personal experiment that could actually change your mind: that is the standard for an n-of-1 design. An experiment designed so that any result confirms what you already believed has not changed anything.

18 likes 4mo
BN
b.nwosuTL2 Moderator29 Mar 2026#49
p.boateng, post #33: Confounding in personal experiments: other things change when you start a medication (season, exercise, diet, stress). Documenting those confounders helps you understand their contribution to the result. Go to post

Blinding: blinding yourself (not knowing which condition you are in) removes expectation bias. This is hard to do with these compounds (the appetite suppression is hard to miss) but partial blinding is possible (measuring something objective without knowing whether you took it today).

0 likes in reply to #33 4mo
MS
m.stephanopoulosTL3Regular1 Apr 2026#50

Designing a personal experiment that could actually change your mind: that is the standard for an n-of-1 design. An experiment designed so that any result confirms what you already believed has not changed anything.

4 likes 4mo
EN
electrolyte_notesTL2Regular3 Apr 2026#51

I read post #49 twice before replying, because I had assumed the opposite.

Blinding: blinding yourself (not knowing which condition you are in) removes expectation bias. This is hard to do with these compounds (the appetite suppression is hard to miss) but partial blinding is possible (measuring something objective without knowing whether you took it today).

24 likes 4mo
YI
y.ibarraTL2 Moderator6 Apr 2026 · edited#52

Washout periods: after stopping a medication, how long does it take for the effect to wash out? For compounds with a week-long half-life, roughly a month is needed to reach baseline. Using that washout period in a before-after design strengthens the inference.

11 likes 4mo
DM
d.moreauTL2Regular8 Apr 2026#53
e.steiner, post #12: Statistical analysis of n-of-1 data: comparing before versus after with a t-test or similar is one approach. Plotting the data visually is another. Both are valid. Go to post

Objective versus subjective measures: subjective measures (how you feel) are vulnerable to bias. Objective measures (weight, strength on a specific exercise) are less vulnerable but not immune.

3 likes in reply to #12 4mo
RL
r.lundgrenTL2 Moderator10 Apr 2026#54

post #53 is right about the mechanism and I think understates the practical bit.

For anyone arriving from a search: the marked solution above is the direct answer, and the replies underneath it add the caveats that make it safe to use.

0 likes 4mo
QZ
q.zhao_qaTL3Quality assurance13 Apr 2026#55

Practical note that does not fit anywhere else. Whatever you conclude from this topic, write down what you did and when. The single most useful thing in your own records is not any individual result; it is that they are dated and consecutive.

32 likes 3mo
SA
s.antonsenTL2 Moderator15 Apr 2026#56

Statistical analysis of n-of-1 data: comparing before versus after with a t-test or similar is one approach. Plotting the data visually is another. Both are valid.

17 likes 3mo
FP
forest_plotTL3Evidence synthesis17 Apr 2026#57
Wendelboe, post #19: Blinding: blinding yourself (not knowing which condition you are in) removes expectation bias. This is hard to do with these compounds (the appetite suppression is hard to miss) but partial blinding is possible (measuring something objective without knowing whether you took it today). Go to post

On post #53 — agreed on the reasoning, with one qualification.

Sample size in n-of-1: you are the sample. Repeated measurements (weekly weighings, daily mood scores) increase the power to detect a real effect even though n=1.

6 likes in reply to #19 3mo
AP
a.pereiraTL220 Apr 2026#58
PE
ppm_errorTL3Analytical chemist22 Apr 2026#59

Generalisability: a robust n-of-1 result applies to you. It does not tell you much about whether the effect generalises to others similar to you, much less to people different from you.

0 likes 3mo
HB
h.bakkerTL2 Moderator24 Apr 2026#60
k.kuusela, post #39: Confounding in personal experiments: other things change when you start a medication (season, exercise, diet, stress). Documenting those confounders helps you understand their contribution to the result. Go to post

This follows post #57 rather than contradicting it.

Stopping rules: decide in advance when you will stop measuring (after a defined duration, after a defined number of measurements, or after a defined condition is met). Not deciding in advance means stopping when the result satisfies you, which is bias.

23 likes in reply to #39 3mo