Tags · Technique
HPLC
High-performance liquid chromatography, the workhorse purity method.
Related in this group: LC-MS · high-resolution MS · MALDI-TOF · UV & DAD detection · peak integration · system suitability · gradient method · water content · amino acid analysis · ELISA · endotoxin testing · sterility testing
48 topics · page 2 of 2
| Topic | Participants | Replies | Views | Activity |
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Detection wavelength and why 214 nm and 280 nm disagree
On the subject in the title: Detection wavelength and why 214 nm and 280 nm disagree Working notes rather than a conclusion. Posting the method first, because I know what the first three replies will…
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+78 | 84 | 1.7k | 8mo |
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Particulates in a reconstituted solution: identifying the likely source
Posting this under the heading it deserves: Particulates in a reconstituted solution: identifying the likely source Everything below is what sits behind that. Posting the method first, because I know what the…
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+15 | 19 | 13k | 9mo |
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Theoretical plates: useful number or ritual?
Theoretical plates: useful number or ritual? I have a specific reason for asking rather than idle curiosity, and the context is below. I would like to understand what this number means before I repeat it…
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2 | 284 | 9mo | |
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Column chemistry choices for a 40-residue peptide
Column chemistry choices for a 40-residue peptide — setting out what I have, and where I think it stops being reliable. Posting the method first, because I know what the first three replies will otherwise be.…
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+39 | 43 | 1.1k | 10mo |
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System suitability injections: if they failed, the run did not happen
Posting this under the heading it deserves: System suitability injections: if they failed, the run did not happen Everything below is what sits behind that. A documentation question rather than an analytical…
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+23 | 27 | 6k | 11mo |
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Reading a chromatogram someone posted without axes
Reading a chromatogram someone posted without axes — setting out what I have, and where I think it stops being reliable. A documentation question rather than an analytical one. I have a certificate in front…
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+47 | 55 | 9.4k | 13mo |
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Area percent versus weight percent: the confusion that causes most arguments — what changed since
Area percent versus weight percent: the confusion that causes most arguments — what changed since Writing it up because I had to work it out twice and would rather nobody else did. Posting the method first,…
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+30 | 34 | 40k | 13mo |
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Particulates in a reconstituted solution: identifying the likely source — what changed since
Particulates in a reconstituted solution: identifying the likely source — what changed since — setting out what I have, and where I think it stops being reliable. Posting the method first, because I know what…
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2 | 129 | 14mo | |
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Residual solvents and scavengers: what is looked for
On the subject in the title: Residual solvents and scavengers: what is looked for Working notes rather than a conclusion. Posting the method first, because I know what the first three replies will otherwise…
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+19 | 23 | 39k | 14mo |
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Coming back to: Oxidation pathways for methionine and tryptophan
Oxidation pathways for methionine and tryptophan Writing it up because I had to work it out twice and would rather nobody else did. Working through the identity arithmetic and I would like it checked.…
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+1 | 5 | 36k | 15mo |
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Deamidation and the close-eluting pair it produces
Deamidation and the close-eluting pair it produces Writing it up because I had to work it out twice and would rather nobody else did. I would like to understand what this number means before I repeat it…
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+33 | 37 | 46k | 15mo |
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Follow-up: Attributing a new peak: degradation product or excipient?
Attributing a new peak: degradation product or excipient? I have a specific reason for asking rather than idle curiosity, and the context is below. Posting the method first, because I know what the first…
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+15 | 19 | 671 | 17mo |
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Attributing a new peak: degradation product or excipient?
Asking directly, because I could not find a straight answer: Attributing a new peak: degradation product or excipient? Posting the method first, because I know what the first three replies will otherwise be.…
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2 | 15k | 18mo | |
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Oxidation pathways for methionine and tryptophan
Posting this under the heading it deserves: Oxidation pathways for methionine and tryptophan Everything below is what sits behind that. Working through the identity arithmetic and I would like it checked.…
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+58 | 63 | 1k | 19mo |
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Why I ask for the gradient before I discuss the number
The question in the title: Why I ask for the gradient before I discuss the number I will give what I have already checked below so nobody repeats it. I would like to understand what this number means before I…
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+35 | 39 | 4.9k | 20mo |
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Follow-up: Trifluoroacetate content and its consequences
On the subject in the title: Trifluoroacetate content and its consequences Working notes rather than a conclusion. Posting the method first, because I know what the first three replies will otherwise be.…
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+12 | 16 | 5.7k | 21mo |
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Gradient steepness and apparent purity: a worked comparison
On the subject in the title: Gradient steepness and apparent purity: a worked comparison Working notes rather than a conclusion. A documentation question rather than an analytical one. I have a certificate in…
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2 | 51k | 22mo | |
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A rising related-substance total over six months: degradation or method drift?
Asking directly, because I could not find a straight answer: A rising related-substance total over six months: degradation or method drift? Posting the method first, because I know what the first three…
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+37 | 42 | 37k | 23mo |