Tags · Technique
LOD & LOQ
Limits of detection and quantitation.
Related in this group: HPLC · LC-MS · high-resolution MS · MALDI-TOF · UV & DAD detection · peak integration · system suitability · gradient method · water content · amino acid analysis · ELISA · endotoxin testing
41 topics · page 1 of 2
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What I would want from a home test that does not exist yet
The question in the title: What I would want from a home test that does not exist yet I will give what I have already checked below so nobody repeats it. A documentation question rather than an analytical…
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+46 | 50 | 8k | 6h |
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Reading a home test result without over-claiming
Reading a home test result without over-claiming — setting out what I have, and where I think it stops being reliable. Working through the identity arithmetic and I would like it checked. retatrutide has a…
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+124 | 137 | 19k | 7h |
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Coming back to: Lateral-flow and colorimetric approaches: the specificity problem
On the subject in the title: Lateral-flow and colorimetric approaches: the specificity problem Working notes rather than a conclusion. I would like to understand what this number means before I repeat it…
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+77 | 89 | 16k | 9h |
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What consumer-accessible tests can and cannot determine — a second dataset
What consumer-accessible tests can and cannot determine — a second dataset I have a specific reason for asking rather than idle curiosity, and the context is below. Posting the method first, because I know…
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+117 | 131 | 45k | 10h |
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Cost versus information: making the trade-off explicitly
Cost versus information: making the trade-off explicitly Writing it up because I had to work it out twice and would rather nobody else did. Working through the identity arithmetic and I would like it checked.…
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+2 | 6 | 12k | 11h |
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A comparison of home results against a laboratory result on the same vial — does this still hold?
A comparison of home results against a laboratory result on the same vial — does this still hold? I have a specific reason for asking rather than idle curiosity, and the context is below. Working through the…
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+2 | 6 | 15k | 11h |
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Reflectance and photometric devices: precision in practice — a second dataset
Reflectance and photometric devices: precision in practice — a second dataset — setting out what I have, and where I think it stops being reliable. I would like to understand what this number means before I…
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+75 | 82 | 17k | 12h |
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Revisiting: Spike-and-recover accuracy for a peptide assay
Revisiting: Spike-and-recover accuracy for a peptide assay — setting out what I have, and where I think it stops being reliable. I would like to understand what this number means before I repeat it anywhere.…
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2 | 6.8k | 21h | |
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What I would want from a home test that does not exist yet — what changed since
Asking directly, because I could not find a straight answer: What I would want from a home test that does not exist yet — what changed since Posting the method first, because I know what the first three…
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+101 | 111 | 1.9k | 21h |
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What a simple presence test is genuinely useful for
What a simple presence test is genuinely useful for — that is the question, and I have not found it answered plainly anywhere I have looked. Working through the identity arithmetic and I would like it…
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2 | 58 | 1d | |
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Revisiting: Can a home kit substitute for a laboratory report? A careful no
Revisiting: Can a home kit substitute for a laboratory report? A careful no — setting out what I have, and where I think it stops being reliable. Working through the identity arithmetic and I would like it…
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+50 | 56 | 9.8k | 1d |
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Specificity, linearity, accuracy, precision, range, robustness — with real criteria
Specificity, linearity, accuracy, precision, range, robustness — with real criteria Writing it up because I had to work it out twice and would rather nobody else did. I would like to understand what this…
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+44 | 48 | 12k | 1d |
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Validating a method you did not develop
On the subject in the title: Validating a method you did not develop Working notes rather than a conclusion. I would like to understand what this number means before I repeat it anywhere. A VendorInvestigate…
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+10 | 14 | 15k | 3mo |
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Sample handling between despatch and injection
Sample handling between despatch and injection Writing it up because I had to work it out twice and would rather nobody else did. Posting the method first, because I know what the first three replies will…
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+63 | 68 | 2.4k | 3mo |
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Second pass at: Robustness: the parameters worth deliberately varying
On the subject in the title: Second pass at: Robustness: the parameters worth deliberately varying Working notes rather than a conclusion. I would like to understand what this number means before I repeat it…
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+61 | 66 | 9.5k | 3mo |
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Spike-and-recover accuracy for a peptide assay
Posting this under the heading it deserves: Spike-and-recover accuracy for a peptide assay Everything below is what sits behind that. A documentation question rather than an analytical one. I have a…
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+121 | 126 | 1.5k | 4mo |
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About the Method validation category
Specificity, linearity, precision, accuracy, robustness, system suitability, and why two labs disagree. This post is a community wiki: any member at trust level 3 or above can edit it, and every edit is…
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+1 | 5 | 468 | 5mo |
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Why one laboratory's 98.4% and another's 96.1% may not contradict — one year on
Asking directly, because I could not find a straight answer: Why one laboratory's 98.4% and another's 96.1% may not contradict — one year on Working through the identity arithmetic and I would like it…
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+10 | 14 | 13k | 5mo |
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Can a home kit substitute for a laboratory report? A careful no
Asking directly, because I could not find a straight answer: Can a home kit substitute for a laboratory report? A careful no I would like to understand what this number means before I repeat it anywhere. A…
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+122 | 131 | 1.7k | 5mo |
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Reading a home test result without over-claiming — what changed since
On the subject in the title: Reading a home test result without over-claiming — what changed since Working notes rather than a conclusion. Working through the identity arithmetic and I would like it checked.…
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2 | 12k | 6mo | |
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Revisiting: Sample handling between despatch and injection
Posting this under the heading it deserves: Revisiting: Sample handling between despatch and injection Everything below is what sits behind that. Posting the method first, because I know what the first three…
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+5 | 9 | 1.1k | 6mo |
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Specificity, linearity, accuracy, precision, range, robustness — with real criteria — a second dataset
On the subject in the title: Specificity, linearity, accuracy, precision, range, robustness — with real criteria — a second dataset Working notes rather than a conclusion. Working through the identity…
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+53 | 58 | 7.5k | 6mo |
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What a stability-indicating method is and how you demonstrate one
Asking directly, because I could not find a straight answer: What a stability-indicating method is and how you demonstrate one Working through the identity arithmetic and I would like it checked. retatrutide…
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4 | 57k | 6mo | |
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Why one laboratory's 98.4% and another's 96.1% may not contradict
Why one laboratory's 98.4% and another's 96.1% may not contradict I have a specific reason for asking rather than idle curiosity, and the context is below. A documentation question rather than an analytical…
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+28 | 37 | 457 | 7mo |
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An inter-laboratory comparison on one lot, tabulated — what changed since
An inter-laboratory comparison on one lot, tabulated — what changed since — setting out what I have, and where I think it stops being reliable. I would like to understand what this number means before I…
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+24 | 28 | 3.3k | 7mo |
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LOD and LOQ: definitions people get wrong
LOD and LOQ: definitions people get wrong — setting out what I have, and where I think it stops being reliable. A documentation question rather than an analytical one. I have a certificate in front of me that…
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2 | 43k | 8mo | |
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Coming back to: Why quantitation is the hard part, not detection
Why quantitation is the hard part, not detection — that is the question, and I have not found it answered plainly anywhere I have looked. Posting the method first, because I know what the first three replies…
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+86 | 92 | 54k | 8mo |
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Lateral-flow and colorimetric approaches: the specificity problem
Lateral-flow and colorimetric approaches: the specificity problem — setting out what I have, and where I think it stops being reliable. A documentation question rather than an analytical one. I have a…
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2 | 4k | 9mo | |
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An inter-laboratory comparison on one lot, tabulated
On the subject in the title: An inter-laboratory comparison on one lot, tabulated Working notes rather than a conclusion. Working through the identity arithmetic and I would like it checked. semaglutide has a…
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3 | 17k | 10mo | |
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Second pass at: LOD and LOQ: definitions people get wrong
Posting this under the heading it deserves: Second pass at: LOD and LOQ: definitions people get wrong Everything below is what sits behind that. Working through the identity arithmetic and I would like it…
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+47 | 51 | 40k | 10mo |
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